摘要
本文考察了基本培养基、碳源、氮源以及有机添加物对霍山石斛原球茎液体培养合成活性多糖的影响。在MS、B5、N6、SH及各自1/2基本培养基中,以1/2MS和N6最适多糖合成,继代培养30d,总多糖产率分别达到479.47mg/L和417.05mg/L。使用单一碳源时,葡萄糖对多糖的积累优于蔗糖和果糖,在30g/L时,总多糖产率达到1034.96mg/L。在一定浓度范围内,氮源种类对多糖的合成影响不太明显,但氮源浓度是主要因素,以10mmol/L最佳。另外,100g/L的马铃薯提取液与100g/L的香蕉提取液均有利于多糖的合成。
Effects of basal media, carbon-source, nitrogen-source and extracts of potato or banana on polysaccharides synthesis of protocorm-like bodies (PLBs) of Dendrobium huoshanense were investigated. Among eight basal media including full- and half- strength of MS, B5, N6 and SH, 1/2Ms and N6 were most suitable for the synthesis of polysaccharides, and the polysaccharide yield reached 479.47mg/L and 417.05mg/L after subculturing for 30d, respectively. For carbon source, it was better in using 30g/ L glucose rather than sucrose and fructose. Under the suitable concentration, the influence of different kinds of nitrogen source on polysaccharide yields was not so big. The optimized nitrogen was at 10mmol/L, while more nitrogen would inhibit the synthesis of polysaccharide. In addition, the addition of extracts of potato (100g/L) or banana (100g/L) to the basal MS medium has benefited the polysaccharide yield.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第4期41-44,共4页
Food Science
基金
教育部科学技术研究重点项目(03098)
关键词
霍山石斛
原球茎
多糖
悬浮培养
Dendrobium huoshanense
protocorm-like bodies (PLBs)
liquid culture
polysaccharides
