摘要
设计一条简便可行的姬松茸发酵液胞外多糖分离路线,采用AKTA explorer100纯化系统,选择DEAE-纤维素柱和Superdex-75柱快速分离纯化。得到主要纯组分为糖蛋白组分FP-1-A,得率为0.58g/L发酵液,性质初步分析表明:该组分相对分子量为54812,紫外扫描表明有蛋白特征吸收峰,红外光谱显示有典型的多糖吸收缝,气相色谱分析其单糖组分由甘露糖和木糖组成。
A brief and feasible system that isolated extracellular polysaccharide from Agaricus blazei Murill ferment liquid was designed. The AKTA explorer with 100 purification system was used with DEAE-cellulose and Superdex-75 to isolate quickly. FP-1-A, a glucan-protein complex, was obtained and its recovery ratio was up to 0.58g per litre ferment liquid. Its analysed property showed that the relative molecular weight was 54812. The UV scan revealed protein characteristic absorbanse apex by and the IR spectrum revealed polysaccharide absorbance apex, and the gas chromatography showed that the components of FP-1-A were composed of mannose and xylose.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第4期75-79,共5页
Food Science
关键词
姬松茸
胞外多糖
分离
纯化
Agaricus blazei Murill
glucan-protein complex
isolation
purification
