摘要
对新月弯孢霉AS3.4381的菌丝体转化16α-甲基Reichstein's化合物S21-醋酸酯(Ⅰ)生成16α-甲基氢化可的松(Ⅱ)进行了研究。培养24h的菌丝体的11β-羟基化活性最高;乙醇对此羟基化活性的抑制作用明显。当(Ⅰ)浓度为0.15%,转化72h,产物(Ⅱ)的重量收率为55.4%。
The conversion of 16α-methyl-Reichstein's compound S 21-acetate(Ⅰ) to 16α-methyl-hydrocortisone (Ⅱ)by the mycelium Curvularia lunata AS3.4381 was studied. Maximal 11β-hydroxylating activity of the mycelium was found during cultivation of the microorganism by 24h. Inhibition of the ethanol to the 11β-hydroxylating activity was obvious. The yield of 55.4%(Ⅱ) (W/W) could be achieved during conversion of 0.15% substrate at 72 h.
出处
《微生物学报》
CAS
CSCD
北大核心
1989年第1期68-71,共4页
Acta Microbiologica Sinica
关键词
新月弯孢霉
羟基化
氢化可的松
Curvularia lunata
11β-hydroxylation
16α-methylhydrocortisone
