摘要
目的 研究Orexin- A m RNA表达在体内主要脏器的分布及在急性炎症损伤后的变化,探讨Orexin- A在急性炎症反应中的作用。方法 取正常雄性SD大鼠和肠缺血再灌注(IR)损伤模型大鼠的下丘脑、胃、肺、肾、附睾脂肪垫、睾丸、肝、脾、十二指肠等主要脏器,采用逆转录聚合酶链反应(RT- PCR)检测Orexin- A m RNA在各主要脏器的分布情况,同时检测IR损伤后下丘脑内Orexin- A m RNA的表达变化。结果 所取各脏器均有Orexin -A m RNA表达,尤其是在胃、肺、肾的表达丰度较高。在IR损伤的急性刺激作用后,下丘脑Orexin- A m RNA表达水平出现波动式的下降趋势。结论 Orexin- A m RNA广泛分布于机体主要脏器,对急性炎症刺激具有时间依赖性的应答效应,其表达水平变化可以作为一种新型的急性炎症反应监测指标。
Objective To explore the distribution of OrexinA mRNA expression in different organs, and its variation in acute inflammation, and to evaluate the role of OrexinA in acute inflammatory responses . Methods Hypothalamus, adipose tissue, lung, stomach, duodenum, liver, spleen and testis of male SpragueDawley rats (including normal and those of intestinal ischemia/reperfusion injury model) were harvested, and reverse transcriptionpolymerase chain reaction(RTPCR) was used to assess OrexinA mRNA expression in different tissues of normal rats, and that in the hypothalamus of ischemia/reperfusion injured rats. Results All tissues expressed OrexinA mRNA, and it was especially high in the stomach, lung and kidney. After the acute stimulus of ischemia/reperfusion injury, OrexinA mRNA expression showed a trend of fluctuant declination in the hypothalamus. Conclusion OrexinA mRNA is widely expressed in the major organs, and it has a timedependent response to acute inflammatory stimuli . The results suggest that its expression variation may be used as a novel marker for acute inflammation .
出处
《中国危重病急救医学》
CAS
CSCD
北大核心
2005年第4期207-210,共4页
Chinese Critical Care Medicine
基金
国家自然科学基金资助项目(39970717)
