摘要
目的 从氧化应激角度探讨硫酸镍致小鼠脾脏脂质过氧化水平和抗氧化能力的变化。方法 硫酸镍0 8,2 0 ,5 0mg/kg小鼠每日腹腔注射染毒 ,连续 30d。制备脾脏组织匀浆 ,采用分光光度法测定脾脏总抗氧化能力(T -AOC)和谷胱甘肽 (GSH)含量 ;黄嘌呤氧化酶法测定超氧化物歧化酶 (SOD)活力 ;用TBA法测定丙二醛 (MDA)含量。结果 镍可抑制小鼠脾脏SOD活力 ,使T -AOC和GSH含量降低 ,MDA含量增加 ,并随染毒剂量增加 ,其效应逐渐增加。结论 硫酸镍可致小鼠脾脏抗氧化能力下降 ,引起脂质过氧化损伤。
Objective To study the effects of nickel sulfate exposure on spleen lipid peroxidation and antioxidant status in mice. Methods Three groups of mice were injected intraperitoneally with nickel sulfate at dose of 0.8,2.5,5.0mg/kg respectively for 30 days to build up the experiment animal model of subchronic toxicity and those in control groups received distilled water at the same time.The spleen activities of superoxide dismutase(SOD)were measured with enzyme assay,meanwhile the contents of total antioxidation capability(T-AOC),glutathione(GSH),malondialdehyde(MDA)were assessed with spectrophotometry.Results The spleen activities of SOD were inhibited by nickel sulfate,meanwhile the spleen levels of T-AOC,GSH were reduced and MDA were increased by nickel sulfate.Conclusion Nickel may drop the antioxidant capability of spleens to cause the damnification of mice spleens.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2005年第2期187-188,共2页
Chinese Journal of Public Health
