摘要
目的 探讨动脉粥样硬化 (atherosclerosis,AS)兔主动脉平滑肌细胞钙激活钾 (Calcium activatedpotassium ,KCa)通道变化及意义。方法 2 0只健康新西兰白兔随机分为AS组和对照组 ,每组 10只。用酶消化法获取单个主动脉平滑肌细胞 ,通过膜片钳记录技术检测AS兔主动脉平滑肌细胞KCa通道的活性 ,并在浴液中分别加入不同浓度的Ca2 + ,实时采样记录通道的开放概率 (Po)、平均开放时间 (To)、平均关闭时间 (Tc)和电流幅值 (Am) ,以加钙前作为对照组观察上述参数的变化。结果 AS组KCa通道的活性明显高于对照组。结论 AS血管平滑肌细胞KCa通道的活性明显增加 ,可能是AS血管发生代偿性扩张的机制之一。
Objective To investigate the changes and significance of calcium-activated potassium(K Ca)channels in aortic smooth muscle cells of New Zealand rabbits with atherosclerosis(AS).Methods Twenty healthy New Zealand white rabbits were randomly divided into two groups,the control group and the AS group.Single aortic smooth muscle cell was obtained by enzymatic treatment. Patch clamp technique was used to record the single K Ca channel current. The signal of channel open probability(Po), mean open dwell-time(To), mean close dwell-time(Tc) and current amplitude were recorded by the software of Pclamp 7.0. After adding different concentrations of Ca 2+ to the bath solution respectively, the above parameters were observed again.Results Compared with that of the control group, the activity of K Ca channel in the AS group was higher.Conclusion There was higher K Ca channel activity in atherosclerotic smooth muscle cells, which was possibly involved in the mechanisms of compensatory vasodilation in AS.
出处
《中华老年心脑血管病杂志》
CAS
北大核心
2005年第1期43-45,共3页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
关键词
动脉硬化
钾通道
钙激活
膜片钳术
肌
平滑
血管
兔
arteriosclerosis
potassium channels,calcium-activated
patch-clamp techniques
muscle,smooth,vascular
rabbits
