摘要
对获取两个杏（ＰｒｕｎｕｓａｒｍｅｎｉａｃａＬ．）品种‘Ｃａｎｉｎｏ’和‘Ｌｕｉｚｅｔ’茎脆散型愈伤组织及建立其细胞悬浮培养进行了研究。结果表明，增加继代培养次数，提高培养基中Ｃａ￣（＋＋）的加入量，选用未木质化的外植体，配以含有较高浓度的２，４－Ｄ和激动素的培养基，能迅速获得１００％的脆散型愈伤组织，成功地建立其细胞悬浮培养系统。
The acquisition of friable calli and the establishment of the cell suspension cultures of the stems of two apricot cultivars( Prunus armeniaca L. cv. canino and Luizet)were studied. The results obtained indieated that 100%friable calli can be acquired rapidly in the presence of higher concentrations of 2,4-D and kinetin in the media,by increasing the number of subeultures,raising the addition of Ca￣(++) to the media and choosing the non-lignified explants,and the cell suspension cultures have been successfully established.
出处
《植物资源与环境》
CAS
CSCD
1994年第3期22-26,共5页
Journal of Plant Resources and Environment
关键词
细胞悬浮培养
愈伤组织
茎
杏
cell suspension cultures
friable calli
stem
Prunus armeniaca L.
