摘要
目的 探索全反式维甲酸在诱导人胚胎神经干细胞(NSC)分化为神经元过程中的作用。方法 用不同浓度(0. 5μmol/L、1μmol/L、5μmol/L和10μmol/L)的全反式维甲酸(ATRA)在体外诱导人胚胎神经干细胞。诱导7d后,做神经元特异性烯醇化酶(NSE)免疫荧光染色,计数分化为神经元的比例。结果 与对照组相比,全反式维甲酸能显著提高人胚胎神经干细胞分化为神经元的比例(P<0. 01)。其中1μmol/L的维甲酸诱导人胚胎神经干细胞分化为神经元的比例最高,为(29. 20±1. 09)%。结论 全反式维甲酸能显著提高人胚胎神经干细胞分化为神经元的比例,其具体机制尚待进一步研究。
Objective To explore the effect of all-trans-retinoic-acid(ATRA) on the course of inducing the human embryonic neural stem cells(NSC)differentiating into neurons.Methods The human embryonic neural stem cells were induced differentiation with different contents of ATRA in vitro. The concentrations of ATRA ranged from 0.5 μmol/L, 1 μmol/L, 5 μmol/L to 10 μmol/L. The immunocytofluorescence was performed seven days later. Quantitation of the neurons was obtained by counting neuron specific enolase(NSE) positive cells. Results The inducing differentiation rates of neurons were improved greatly by ATRA compared with control (P<0.01). The concentration of 1 μmol/L ATRA was the most suitable for inducing differentiation of the human embryonic neural stem cells among the experimental groups, and the percentage of differentiated neurons was (29.20±1.09)% by 1μmol/L ATRA.Conclusion ATRA can increase greatly the percentage of neurons in the course of inducing the human embryonic neural stem cells differentiation, while, its mechanism will be investigation in the future.
出处
《中华神经外科疾病研究杂志》
CAS
2005年第2期145-148,共4页
Chinese Journal of Neurosurgical Disease Research
