摘要
目的 了解丙型肝炎病毒(HepatitisCvirus, HCV) 无症状感染者病毒载量分布以及与ALT水平的相关性。方法 采用荧光定量PCR检测80例HCV无症状感染者外周血中HCVRNA, 同时进行重组免疫印迹试验和血清丙氨酸转氨酶(ALT) 水平测定。结果 80例HCV无症状感染者外周血HCVRNA含量在102 1 ~105 8拷贝/ml, 其中82.5% (66 /80) 在104 0 ~105 8拷贝/ml。RIBA不同反应组HCVRNA含量间差异无显著性意义(P>0.05), ALT异常升高组HCVRNA含量与ALT正常组之间差异无显著性意义(P>0.05)。结论 HCV无症状感染者病毒载量处于相对低水平, 病毒载量与RIBA抗体反应模式和ALT水平的关系有待进一步研究。
Objective In order to know the interrelation of serum ALT level in hepatitis C viral RNA in sera of asymptomatic blood donors. Methods The concentrations of HCV RNA were detected in 80 asymptomatic blood donors by quantitative polymerase chain reaction which was based on an Amplisensor assay. HCV antibody in sera was further detected by RIBA-3 and the serum ALT level was also detected.Results The amounts of HCV RNA ranged from 10^(2.1)copies/ml to 10^(5.8)copies/ml in sera from 80 asymptomatic blood donors and 82.5%(66/80)was in 10^(4.0)～10^(5.8)copies/ml. There was no obvious difference between the amounts of HCV RNA and present of HCV-antibody and serum ALT level (P<0.05).Conclusion The viral level of hepatitis C viral RNA in sera of asymptomatic blood donors was low. The correlation between the quantity of HCV RNA and present of HCV-antibody and serum ALT level needs to be further studied.
出处
《中国全科医学》
CAS
CSCD
2005年第7期556-557,560,共3页
Chinese General Practice
