摘要
目的研究功能未知新基因LX3[1]与白细胞介素-6(IL-6)的相关性,为研究IL-6的作用机制寻找新的靶基因。方法反转录-PCR(RT-PCR)分析不同浓度IL-6处理的U937细胞及同一浓度IL-6诱导不同时间的U937细胞中新基因LX3的表达差异;NorthernBlot分析IL-6诱导前后U937细胞中新基因LX3表达的变化。结果LX3基因在U937细胞中的表达量随IL-6诱导浓度的增加而增加,在IL-6浓度为500ng/ml时表达量最高,而在0ng/ml时不表达;时间表达谱分析显示在IL-6刺激8h时新基因LX3的表达量最高;Northern印迹分析显示新基因LX3在IL-6诱导后的U937细胞中的表达量明显升高。结论LX3是与IL-6诱导紧密相关的新基因。
Objective To analyze the association between the expression of the novel gene LX3 and interleukin-6 (IL-6) induction, and explore a new target gene for action mechanism of IL-6. Methods The total RNA was extracted from U937 cells induced by IL-6 at different concentrations and varied lengths of time. Reverse transcriptional (RT)-PCR and Northern blotting were employed to determine the expression of LX3 and IL-6 induction. Results The expression of novel gene LX3 was increased while concentration of IL-6 was improved gradually. High expression was induced by IL-6, which was highest at the concentration was 500 ng/ml and no expression at 0 ng/ml. Time- expression pattern proved that expression of novel gene LX3 of higest at 8 hour after induced by IL-6. Northern blotting confirmed that the expression quantity of LX3 increased in U937 cells induced by IL-6. Conclusion The quantity of LX3 expression is associated with the dose and duration of IL-6 induction, suggesting that LX3 gene is a novel gene correlated with IL-6 induction.
出处
《第一军医大学学报》
CSCD
北大核心
2005年第4期384-386,共3页
Journal of First Military Medical University
基金
国家杰出青年科学基金(39925019)~~
关键词
白细胞介素-6
LX3
反转录-PCR
interleukin-6
LX3
reverse transcriptional polymerase chain reaction
