摘要
目的确定复方蒲公英灌肠液中的活性成分。方法利用三维高效液相色谱对复方蒲公英灌肠液的乙酸乙酯部分分离分析,采用液相色谱‐质谱联用技术对其色谱图谱进行确定。结果通过质谱的质荷比(M/Z)及色谱保留时间的相互比较,确定了咖啡酸、阿魏酸和原儿茶醛3种活性成分。结论液质联用技术可用于复方蒲公英灌肠液的定性分析。
Objective To identify the active components of compound dandelion enema, a preparation from 7 traditional Chinese herbal drugs for treatment of gynecological diseases. Methods Three-dimensional high-performance liquid chroma- tography (3D-HPLC) was employed to separate the ethyl acetate extract of compound dandelion Enema, and HPLC combined with mass spectrum (MS) analysis used for chromatographic fingerprinting. Result By comparing the ionic fragments of MS and retention time of each peak, the main active components in compound dandelion enema were determined, including caffeic acid, ferulic acid and protocatechualdehyde. Conclusion HPLC coupled with mass spectroscopy can be used for qualitative analysis of compound dandelion enema.
出处
《第一军医大学学报》
CSCD
北大核心
2005年第4期447-449,453,共4页
Journal of First Military Medical University
关键词
高效液相色谱
液质联用
复方蒲公英灌肠液
high-performance liqid chromatography
mass spectroscopy
compound dandelion enema
