摘要
①目的 比较测定不同产地苦参中生物碱的含量。②方法 采用高效液相色谱法测定苦参中生物碱的含量。色谱条件: 氨基键合相柱,乙睛-磷酸水溶液(pH=2)-无水乙醇(8∶1∶1)为流动相,检测波长220nm。③结果 苦参碱、氧化苦参碱和槐定碱 回收率分别为98.90%、98.94%、100.6%。RSD分别为1.83%、1.85%、1.24%。④结论 采用氨基键合相柱测定苦参中苦参碱、氧化 苦参碱和槐定碱,方法简便易行,准确度高。
Objective To determined the content of matrine,oxymatrine,sophoridine in Kushen from different area. Methods To determined content of alkaloid in Kushen with HPLC,Amino bonded-phase column was used with mobile phase acetonitrile-phosphoric acid-absolute ethyl alcohol (8∶1∶1),the wavelength of determination was 220nm.Results The average recovery of matrine,oxyumatrine and sophordine were 98.90%,98.94%,100.6%.RSD were1.83%,1.85%,1.24%. Conclusion To determined content of alkaloid in Kushen with amino bonded-phase column was simple and reliable.The accuracy of determination was high.
出处
《华北煤炭医学院学报》
2005年第2期140-141,共2页
Journal of North China Coal Medical College
