摘要
目的 了解上海市透析用水、透析液的细菌污染状况。方法 采集上海市 10家医院反渗水及透析液标本 ,对反渗水及透析液标本进行细菌培养和菌落计数 ;用LAL试剂盒测定反渗水及透析液中的内毒素含量。结果 反渗水细菌培养有 3家医院阳性 ,细菌计数均 <2 0 0CFU/ml,内毒素为 (0 .5 19± 0 .6 87)EU/ml,其中 5家 >0 .2 5EU/ml;A浓缩透析液内毒素为 (0 .2 35± 0 .2 2 7)EU/ml;B浓缩透析液内毒素为 (0 .6 88± 0 .74 2 )EU/ml;透析液入口细菌培养 3家阳性 ,其中 1家 >2 0 0 0CFU/ml,内毒素为 (1.4 4 9± 3.4 73)EU/ml,其中 1家 >5EU/ml。结论 目前上海市部分血液净化单位仍存在反渗水及透析液的细菌污染 ,应定期检测运血导管、透析器、透析机和透析液的污染情况 ,并定期消毒。
Objective To evaluate the bacterial contamination i n reverse osmosis (RO) water and dialysis fluids using for hemodialysis treatmen t in Shanghai. Methods Specimens of reverse osmosis water and dialysis fluids were obtained from 10 hemodialysis centers in Shanghai. Bacterial culture was pe rformed and endotoxin (ET) was measured with chromogenic limulus amoeboeyte lysa te (LAL) assay. Results The level of ET in RO water was (0.519±0.687)EU/ml, and there were 3 positive results of bacteria culthure, both less than 200 CFU/m l. The level of ET in dialysis inlet was (1.449 ± 3.473)EU/ml with 1 sample h igher than 5 EU/ml, and the culture of bacteria had 3 positive results, with 1 s ample higher than 2000 CFU/ml. Conclusion Bacterial contamination exist in RO water and dialysa te in shanghai hemodialysis center. It is essential to examine and disinfect RO and dialysate tank at regular intervals.
出处
《中国血液净化》
2005年第1期52-54,共3页
Chinese Journal of Blood Purification
关键词
血液透析
透析液
致热原
内毒素
Hemodialysis, Dialysis fluids, Pyrogen, Endotoxin
