摘要
目的 分离纯化库拉索芦荟多糖并建立含量测定方法。方法 醇沉法提取库拉索芦荟多糖,SephadexG 100凝胶柱色谱纯化。以甘露糖为标准,苯酚硫酸法测定库拉索芦荟多糖含量。结果 经分离纯化得到的主要组分库拉索芦荟多糖I含量为81. 11%,测定平均回收率为100. 3%。结论 该方法可从库拉索芦荟中提取分离纯度较高的多糖,含量测定方法简便、快速、重现性好。
OBJECTIVE To isolate, purify the polysac ch aride of Aloe barbadensis Miller and establish the content determination method.METHOD The polysaccharide was precipitated with ethanol and fur ther purified on Sephadex G-100 column. Its total soluble sugar content was det ected colorimetrically by the phenol-sulfuric acid method with mannose as stand ard. RESULTS The total sugar content of polysaccharide I was 81.11%. The average recovery was 100.3%. CONCLUSION It is possible to extract and isolate purified polys accharide from Aloe barbadensis Miller. And the determination method is simple, rapid and well reproducible.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2005年第2期131-133,共3页
Chinese Journal of Modern Applied Pharmacy
基金
福建省科技厅重点攻关课题(2002I015)
关键词
库拉索芦荟
多糖
分离
纯化
测定
Aloe barbadensis Miller
polysaccharide
isolatio n
purification
determination
