摘要
采用均匀设计法优化嗜酸乳酸杆菌的发酵培养基, 研究一株可作为益生素使用的嗜酸乳酸杆菌CGMCC0842的生物学特性, 包括菌体生长曲线、耐热存活率、耐酸存活率、贮藏存活率等. 结果表明, 嗜酸乳酸杆菌的活菌浓度在开始时缓慢上升, 3h后从105 CFU/mL迅速上升, 第18h超过1011 CFU/mL, 到第32h, 细菌数量则缓慢下降, 菌体的适宜收获期宜在发酵后18~23h. 嗜酸乳酸杆菌的75℃处理15min耐热存活率为62.5%, 贮藏1个月的存活率为59 %, pH2.0处理6h的存活率69.5%. 优化的发酵参数为: 时间, 20h; 葡萄糖, 110g/L; 蔗糖, 10g/L; 胰蛋白胨, 5g/L; 酵母浸粉, 30g/L; 柠檬酸盐, 12g/L. 研究表明该乳酸杆菌CGMCC0842是一株优良的饲用益生素菌种.
Some important characteristics of Lactobacillus acidophilus CGMCC0842 were investigated in vitro to provide data for application in feed additive.The growth curve,heat tolerant survivabillity,acid tolerant surviability,storage survivability and fermentation parameters were studied in this experiment.Fermentation parameters experiment was optimized according to the uniform design,in which there is much less experiment required.The concentration of Lactobacillus acidophilus increased slowly at the beginning.After (3 hours) the concentration grew very quickly from 10~5 CFU/mL to 10^(11) CFU/mL. The survivability of Lactobacillus acidophilus of heat resistance(75 ℃,15 min),acid resistance(pH2.0, 6 h) and storage(1 month)is 62.5 %,69.5%, and 59 % respectively.The optimal fermentation parameter was as follows:culture time, 20 h; glucose, 110 g/L; sucrose,10 g/L;peptone 5 g/L; yeast,30 g/L, and ammonium citrate, 12 g/L. The results showed that this strain of Lactobacillus acidophilus was a good potential strain for feed probiotic preparation.
出处
《集美大学学报（自然科学版）》
CAS
2005年第1期23-28,共6页
Journal of Jimei University：Natural Science
基金
国家"十五"攻关"生物饲料添加剂研究与开发" (2002BA514A-12)
集美大学引进人才科研启动基金 (C60311)
关键词
嗜酸乳酸杆菌
生物学特性
发酵参数
Lactobacillus acidophilus
biological characteristics
fermentation parameters
