摘要
目的:观察病毒唑体外对副流感病毒( 型)的抑制作用。方法:采用细胞病变抑制实验,观察病毒唑在Hela细胞中对副流感病毒( 型)的抑制作用。结果:病毒唑半数中毒浓度(TC50 )为1783.86μg/ m l;抗副流感病毒( 型)的半数有效浓度(EC50 )为11.5 3μg/ ml,治疗指数(TI)为15 4 .71;病毒唑对副流感病毒( 型)的抑制作用存在明显的量效反应关系(P<0 .0 1) ;在感染病毒后0～2 4 h,每隔2小时加一次药,病毒唑的5 0、2 5、12 .5、6 .2 5 μg/ ml组对副流感病毒( 型)均有抑制作用(P<0 .0 1) ;病毒唑对副流感病毒( 型)有预防和中和作用(P <0 .0 1)。结论:病毒唑在Hela细胞中对副流感病毒( 型)有明显的抑制作用,其作用机制是多途径的。
Objective: To assess the inhibitory effect of Rib av irin on parainfluenza viruses(Ⅲ). Methods: The cytopathic effect inhibition assay was used to test the antiviral activity of Ribavrin on parainf luenza viruses(Ⅲ) in Hela cells. Results: Ribavirin was an effe ctive antiviral agent for parainfluenza viruses(Ⅲ) in a concentration-dependent manner and its median toxic concentration(TC 50), median efficacious conce ntration(EC 50) and treatment index(TI) was 1783.86μg/ml,11.53μg/ml and 154.71, respectively. Ribavirin at the concentrations of 50, 25, 12.5 and 6. 25μg/ml exhibited an inhibitory effect (P<0.01)when added into cell cultu re 0 to 24h following virus infection at every 2h. The parainfluenza viruses(Ⅲ) were prevented and neutralized by Ribavirin. Conclusion: Ribavi rin shows a significant inhibitory effect on parainfluenza viruses(Ⅲ) in multip le ways.
出处
《黑龙江医药科学》
2005年第2期5-7,共3页
Heilongjiang Medicine and Pharmacy
