摘要
208对引物中具有多态性的引物123对,占所用引物的59.13%.不同染色体的微卫星分析的多态性不同,染色体9,10微卫星的多态性高于其它染色体,染色体12上的微卫星标记的多态性最差,仅为46.15%.聚类分析表明,所有的供试材料可分为两群,即籼稻群和粳稻群.聚类结果与亲本材料亲缘关系基本一致,说明微卫星标记能较好地区分籼稻和粳稻.由于农艺性状是基因表达的结果,易受环境影响,聚类结果不能从整体上充分反应品种间的遗传变异.42份常用杂交水稻亲本材料聚类分析表明,恢复系和不育系遗传基础均较狭窄,但恢复系和不育系之间的遗传距离相对较远,从一定程度上反映了遗传距离与杂种优势正相关.
123 primers of 208 have polymorphism. The results showed significantly higher genetic diversity on chromosome 9 and 10 and considerably lower diversity on chromosome 12. Through clustering on SSR primers, the relationship and hereditary base of 93 varieties and 42 parents of hybrid were evaluated in the project. All materials used in the study were clustered into indica group and japonica group which are generally agreed with pedigree analysis. The results indicated that SSR markers play an important role in variety identification and study of genetic diversity.
出处
《山东理工大学学报(自然科学版)》
CAS
2005年第2期22-27,共6页
Journal of Shandong University of Technology:Natural Science Edition