摘要
目的分析人苍白杆菌的耐药性及AmpC酶。方法回顾性分析2001年至2003年我院分离到的83株人苍白杆菌耐药性,并对2004年2月保存的8株菌株,采用浓度梯度法测定12种抗菌药物的最低抑菌浓度,三维试验分析β内酰胺酶及PCR扩增耐药基因,脉冲场凝胶电泳技术(PFGE)分析同源性。结果83株人苍白杆菌均来自肝移植患者的血液标本,对大多数β内酰胺类抗生素有很强的耐药性,但对喹喏酮类、碳青霉烯类和氨基糖苷类抗菌药物有较好的敏感性,敏感率均在80%以上。8株人苍白杆菌PFGE条带完全一致,来自同一克隆株,且均产AmpC酶,与OCH-4酶(CAC17624)同源性最高为99%。结论人苍白杆菌对大多数头孢菌素和青霉素类抗生素有很强的耐药性与其产AmpC酶有关。
Objective To analysis the antibiotic resistance and AmpC β-lactamase of Ochrobactrum anthropi.Methods Analyzing the antibiotic resistance of 83 clinical strains of O. anthropi isolated in our hospital from 2001 to 2003.Etest was made to determine the minimal inhibitory concentration(MIC) of the eight reserved clinical strains isolated in February , 2004 . The β-lactamases produced by these ioslates were typed by three-dimensional extract method.The polymerase chain reaction(PCR) was performed to amplify the entire coding sequence of the O. anthropi ampC and ampR gene. The homology of these isolates was also analyzed by pulsed-field gel electrophoresis (PFGE). Results All of O. anthropi isolates were obtained from blood culture of liver transplant patients.O. anthropi was resistant to most of β-lactams, but susceptible to quinolones, carbapenems and aminoglycosides(>80%). 8 cilinal isolates had the same PFGE pattern and produced AmpC β-lactamase.The deduced amino acid sequence of the AmpC β-lactamase was 99% identical to the sequence of OCH-4(CAC17624).Conclusion O. anthropi was resistant to the most cephalosporins and penicillins due to the expression of the AmpC β-lactamase.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2005年第4期378-380,共3页
Chinese Journal of Laboratory Medicine
基金
国家自然科学基金资助项目(30370073)
