摘要
目的:应用三色流式细胞术研究儿童急性淋巴细胞白血病(AL L)免疫表型特征。方法:采用CD4 5/ SSC双参数散点图设门方法检测37例儿童AL L 免疫表型。结果:37例儿童AL L 中B- AL L 34例,占91.9% ,其中CD1 9阳性表达率10 0 % ,明显高于CD1 0 (82 .4 % ,P<0 .0 5 )和CD2 2 (6 1.8% ,P<0 .0 1)的表达。T- AL L 2例,仅占5 .4 % ;CD34 /HL A- DR阳性率较高(86 .5 % / 81.1% ) ,二者表达较一致;AL L 伴髓系抗原表达占2 7% ,主要表达CD33。结论:儿童AL L中以B- AL L为主,B- AL L白血病细胞稳定持续表达CD1 9,CD1 9是诊断B- AL L较为可靠的表面标记。此外,儿童AL L具有较高的CD34 和髓系抗原表达。
Objective:To study the character of immunophenotype s in childhood acute lymphoblastic leukemia(ALL) using three-color flow cytometry.Methods:Immunophenotyping assay for 37 cases of childhood ALL was performed by FCM analysis using CD 45 /SSC gating.Results:Among 37 cases of ALL,34 cases (91.9%) were B-ALL,in which the expression of CD 19 was 100%,much higher than those of CD 10 (82.4%,P<0.05) and CD 22 (61.8%,P<0.01).Twenty-seven percent of all patients was expressed with myeloid-associated antigen mainly including CD 33 .Conclusion:B-ALL is the major subtype in childhood ALL.CD 19 ,which is expressed stabley in B lineage ALL,is reliable cell membrane marker for the diagnosis of B-ALL.Relatively higher expression of CD 34 and myeloid-associated antighen were observed in childhood ALL.
出处
《临床医药实践》
2005年第4期266-267,共2页
Proceeding of Clinical Medicine
