摘要
用五段分级采样器采集太原某焦化厂厂区大气颗粒物,用液相色谱测定不同粒径颗粒物上5种多环芳烃(PAH)的含量,并用Ames试验及CHL细胞姐妹染色单体互换(SCE)试验,观察不同粒径颗粒提取物的致突变性。结果表明5种PAH浓度均很高,芘为14.89μg/100m3、为19.35μg/100m3、苯并(a)蒽(BaA)为18.9μg/100m3、为114.67μg/100m3、苯并(a)芘(BaP)为15.9μg/100m3。47~70%的PAH富集在≤2μm细颗粒上,其质量中值直径芘、、BaA、、BaP分别为2.2、2.1、1.6、1.9、1.3。Ames试验和SCE试验结果均表明,分级颗粒粒径愈小其提取物致突变作用愈强。
Airboren particles samples were collected at coking plantin Taiyuan using five-stage classification air samples. Theconcentratiom of five PAHs were determined by the high performance liquid chromatography. The mutagenicity was studied by the Ames assay and SCEs test in CHL cells. The results indicated the concentration of five PAHs in particleswere high. 47-70 percent PAHs were concentrated on theparticles≤μm. MMD of them were 2. 2 (Pyrene), 2. 1 (Chrysene). 1. 6 (Benz(a) anthracene), 1. 9 (Perylene)and 1. 3 (Benzo (a) pyrene). The results in Ames assay and SCEs testshowed that all the extracts from various size airborne particles were mutagenicity and the smaller the st;. of particleswas, the stronger the mutagenicity induced was.