摘要
应用人外周血淋巴细胞的非程序ＤＮＡ合成试验（ＵＤＳ）和小鼠ＤＮＡ合成抑制试验（ＤＳＩ），对南四湖污染严重的沙堤、薛排沟、下航道三个监测点水中有机提取物，进行致突变活性检测。结果显示三个监测点水样有机提取物均可诱发ＣＰＭ值显著性增加；实验组小鼠肝、肾和睾丸组织的３Ｎ－ＴｄＲ掺入量明显下降，与阴性对照组相比有显著性差异，对ＤＮＡ合成抑制呈明显剂量一效应关系。结果还显示南四湖水中有机污染物主要是ＤＮＡ损伤剂。
The unscheduled DNA synthesis(UDS) and the inhibition of whole mouse DNA synthesis test(DSI). Were used to detect the mutagenicity of water organic extract substances(WOES) from Nan-Si Lake.The main index that differentiates the damage of DNA matter was inhibition of mouse testicular DNA synthesis. The results show that IVOES from Nan-Si Lake can increase the CPM of lymphocyte apparently; it has significant inhibition for DNA synthesis of liver. Kidney and testis; and there is a significant dose-effect relationship. These suggest that there are mutagens gausing damage of DNA matter from Nan-Si Lake water.
出处
《中国环境科学》
EI
CAS
CSSCI
CSCD
北大核心
1994年第3期210-213,共4页
China Environmental Science
