摘要
对蜡质芽孢杆菌(Bacilluscereus)DLSL2深层液体发酵的主要影响因子温度、转速、初始pH值等进行了单因素实验探讨,确定了最佳培养条件:温度为30℃、转速为250r/min、初始pH值为7.0。并用均匀设计法对其发酵培养基进行了优化,优化验证实验结果为7.1×109cfu/mL明显高于原发酵培养基结果3.2×109cfu/mL。
The fermentation of Bacillus cereus DLSL-2 was investigated through single-factor test. The optimized fermentation conditions are: temperature 30℃,initial pH 7.0,250 r/min. Uniform design was used in shaking flask to optimize the fermentation medium of bacillus cereus . The most suitable medium was identified as follows:4.88% defated soybean power ,1.45% maize starch, 0.106% K 2HPO 4, 1.78% yeasts extract, 5.6% inoculum. the optimized medium allowed the B.cereus biomass concentration to be increased from 3.2×109 cfu/mL to 7.1×109 cfu/mL.
出处
《微生物学通报》
CAS
CSCD
北大核心
2005年第2期45-49,共5页
Microbiology China
基金
2003天津市科技发展计划项目
关键词
蜡质芽孢杆菌
发酵
培养基优化
均匀设计
Bacillus cereus, Fermentation, Medium optimization , Uniform design
