摘要
目的:探讨归参胶囊对乳腺增生病模型大鼠血清中雌二醇、孕酮、催乳素的影响。方法:实验于2004-05/07在三峡大学医学院机能实验室完成。清洁级雌性未孕SD大鼠48只,体质量(200±20)g。随机分为6组,每组8只。①正常对照组:实验过程中正常饲养,不作处理。②模型组:灌服生理盐水5mL/(kg·d)。③低剂量2.1g/(kg·d)归参胶囊组。④中剂量4.2g/(kg·d)归参胶囊组。⑤高剂量8.4g/(kg·d)归参胶囊组。⑥夏枯草口服液组2.1mL/(kg·d)。除正常对照组外,各组均腹腔注射苯甲酸雌二醇复制大鼠乳腺增生模型,同时应用相应药物治疗,1次/d,连续用药4周后停止注射苯甲酸雌二醇,继续灌服上述各种药物2d。于末次给药后处死全部大鼠,应用放射免疫法检测血清中雌二醇、孕酮、催乳素的含量;同时取乳腺组织作切片观察。结果:48只大鼠均进入结果分析。①血清雌二醇、催乳素水平:模型组较正常对照组含量显著升高(68.28±57.61)ng/L,(3.59±0.35)μg/L,(16.06±2.36)ng/L,(2.87±0.40)μg/L,两组比较数据差异有明显意义(P<0.05或0.01);而低、中、高剂量归参胶囊组和模型组相比雌二醇、催乳素含量明显降低(14.97±4.92)ng/L,(2.95±0.53)μg/L,(11.56±4.00)ng/L,(2.75±0.61)μg/L,(15.50±3.43)ng/L,(2.39±0.67)μg/L,(P<0.05或0.
AIM:To investigate the therapeutic effect of guishen capsule on serum concentr ation of estradio(E2),progesterone(P) and prolactin(PRL) in rat models of mammar y gland hyperplasia. METHODS:The experiment was completed in the Function Laboratory of College of Medical Sciences,China Three Gorges University from May to July 2004. Forty eig ht unfertilized female SD rats in clean grade weighing (200±20) g were randomly divided into 6 groups with 8 rats each. ①Rats in the control group were fed no rmally without any treatment;②The rats in model group were infused with normal saline 5 mL/kg per day;③The rats in the low dose guishen group were treated wi th guishen 2.1 g/kg per day;④The rats in middle dose guishen group were with g uishen 4.2 g/kg per day;⑤The rats in high dose guishen group were with guishen 8.4 g/kg per da;⑥The rats in oral solution group were treated with common self heal fruit spike(a kind of Chinese herb) oral solution 2.1 mL/kg per day. The m odels of mammary gland hyperplasia were induced by benzoate estradiol intraperit oneal injection in SD female rats. Benovocylin was injected into the abdominal c avity to copy the models of rat mammary gland hyperplasia in all the groups exce pt the control group,and all the groups received corresponding treatment once pe r day for consecutive 4 weeks.At the end of 4 weeks,benovocylin was quitted to i nject,and corresponding medication continued for another two days. All rats were killed after the last medication to draw blood from hearts for the determinatio n of serum E2,P and PRL level with radioimmunoassay(RIA). Meanwhile,the tissue s ection of mammary glands was taken out and observed. RESULTS:All the 48 rats finished the result analysis. ①The level of serum E2 and PRL was significantly higher in the model group than in the control group[(6 8.28±57.61) ng/L,(3.59±0.35) μg/L vs(16.06±2.36) ng/L,(2.87±0.40) μg/L](P< 0.05 or 0.01);That in the low ,middle and high dose guishen group[(14.97±4. 92) ng/L,(2.95±0.53) μg/L,(11.56±4.00) ng/L,(2.75±0.61) μg/L,(15.50±3.43) ng/L,(2.39±0.67) μg/L respectively] was significantly lower than that in the m odel group(P< 0.05 or 0.01).②The number of mammary lobule and acinus was increa sed,lumen of catheter and alveolar cavity were obvioulsly enlarged in the model group. In the guishen capsule treatment groups,the number of mammary lobule and acinus was decreased to different extents,a large number of acinus was at break up and degradation,and lumen of catheter was contracted. CONCLUSION:Guishen capsule has obvious effect of decreasing the serum level of E2 and PRL. It can inhibit hyperplasia of mammary gland and regulate the endocr ine hormone balance.
出处
《中国临床康复》
CSCD
北大核心
2005年第14期162-163,共2页
Chinese Journal of Clinical Rehabilitation
