摘要
用^(32)P标记含间日疟原虫DNA片段的重组质粒pVA1作为探针,通过DNA打点杂交试验检测红内期间日疟原虫。该探针检测间日疟原虫基因组DNA的敏感度达1ng;与现场采集的25份间日疟患者血样(原虫血症为0.003％～0.7％)的杂交阳性率为72％,与6例恶性疟和3例食蟹猴疟血样中各1例杂交阳性;与3例约氏疟和6例正常人血样均为阴性。
With DNA Dot blot hybridization assay,clinical malaria blood samples were examined using 32P-labelled recombinant plasmid pVAl,which contains DNA fragment of P. vivax Sensitivity of the probe detecting P. vivax genomic DNA was about Ing; hybridized positive rate of microscopically diagnosed P. vivax samples (parasiteamia from 0. 003% to 0. 7%) was 72% (18/25) ; A group of 6 P. falciparum samples and a group of P. cynomologi samples each had 1 positive sample detected, but no cross-reaction with 3 P.yoelii samples and 6 normal human blood samples was moted. The result suggests that recombinant plasmid pVAl DNA could be used as aprobe for preliminary detection of P. vivax .
出处
《中国寄生虫病防治杂志》
CSCD
1994年第1期7-9,共3页
Chinese Journal of Parasitic Disease Control
