摘要
目的:研究中药益髓生血颗粒治疗β-地中海贫血(β-地贫)的临床效果及其疗效特点的分子机制。方法:用补肾益髓法代表方益髓生血颗粒在广西高发区治疗β-地贫92例,疗程3个月,观察治疗前后临床症状,血红蛋白(Hb)、抗碱血红蛋白(HbF)、红细胞(RBC)、网织红细胞(Ret)等血液参数变化及停药后随访观察,采用 PAGE、PCR-SSCP、RT-PCR、DNA 序列分析、mRNA 基因表达、DDRT-PCR 等技术研究疗效的分子机制。结果:经3个月治疗后,患者临床症状明显改善,Hb、HbF、RBC、Ret 等血液指标明显提高,停药后疗效平均维持3～6个月,92例中85例有效,有效率92.39%;中药治疗对各类基因型患者均有一定疗效,可明显提高血红蛋白珠蛋白 mRNA 表达和造血刺激因子 GM-CSFmRNA 表达,诱导 HbF 合成增加;降低铁蛋白基因表达,减少体内铁的蓄积。结论:益髓生血颗粒治疗β-地中海贫血效果显著;中药治疗不改变患者基因突变型,可明显调节功能基因表达。疗效特点为多靶点的整体效应。
Objective:To study clinical effect and molecular mechanisms of Yisui Shengxue Granules for treatment of β-Miditerranean anemia.Methods:92 cases of β-Miditerranean anemia in high morbidity regions of Guangxi were treated with Yisui Shengxue Granules,a representative recipe of the method for tonifying kideny and supplementing marrow,for 3 months.Clinical symptoms and signs,hemoglobin(Hb),alkali-resistant hemoglobin(HbF),red blood cell(RBC),reticulocytes(Ret)etc.before and after treatment were observed and followed up.The molecular mechanisms were studied with PAGE,PCR-SSCP,RT-PCR,DNA sequential analysis,mRNA gene expression,DDRT-PCR and so on.Results:After treatment for 3 months,the symptoms improved significantly,Hb,HbF,RBC,Ret increased significantly,and the therapeutic effects lasted for 3～6 months after ceasing the treatment.Of the 92 cases,85 cases were effective,the effective rate being 92.39%.The medical treatment had a certain therapeutic effect for the patients of various gene types,and could significantly increase hemoglobin and globin mRNA and GM- CSFmRNA expressions,increase synthesis of HbF,decrease iron protein gene expression and accumulation of iron in the body. Conclusion:Yisui Shengxue Granules has a significant effect on β-Miditerranean anemia,and it does not change gene mutation type of patients and can significantly regulate expression of functional genes,with the characteristic of whole effect of multiple targets.
出处
《中医杂志》
CSCD
北大核心
2005年第4期277-279,共3页
Journal of Traditional Chinese Medicine
基金
国家自然科学基金(30171199)
广西自然科学基金(014402C)
国家自然科学基金重大研究计划(90409003)
