摘要
目的:探讨尼古丁与癌基因表达和膀胱癌生物学行为的关系。方法:对40 只大鼠以10% BBN为致癌剂膀胱灌注诱发膀胱癌,其中30只分25、15、5 mg/kg剂量以咽管灌胃方式给予尼古丁, 10 只作为对照,应用免疫组化方法对膀胱癌模型中p53蛋白进行检测。结果:不同剂量的尼古丁干预下的p53 蛋白表达的阳性率分别为60%、30%、20%。p53蛋白表达阳性率与给药剂量、给药时间呈正相关。结论:尼古丁对癌基因的异常表达及协同作用,在膀胱癌的发生发展中起一定作用。
Objective:Purpose: To explore the relationship between nicotine and the expression of oncogenes of bladder carcinoma and its biological characteristic. Methods:The expression of p53 proteins in 40 cases bladder carcinoma model of wistar rats was determined with immunohistochemistry. Results:The positive rate of expression for p53 proteins under the influence of nicotine in various dose were 60%, 30% and 20% respectively. The positive rate of expression for p53 proteins was positively correlated to medication dose and time. Conclusions:Abnormal expression and synergistic action of oncogenes and nicotine play an important role in occurrence and development of bladder carcinoma.
出处
《临床泌尿外科杂志》
2005年第5期301-303,共3页
Journal of Clinical Urology
