摘要
目的 探讨免疫透射比浊法对血清单克隆免疫球蛋白(Ig)异常高值定量的问题。方法 采用HITACHI71 70 A全自动生化分析仪5点定标透射比浊法与Beckman特定蛋白分析系统速率散射比浊法,测定30例免疫球蛋白(Ig G)异常高值血清。结果 两法在测定血清单克隆异高值Ig G时结果差异具有显著性(P<0 .0 1 ) ,一定浓度范围内(低高、中高组)相关系数r=0 .96 6 ,定量正常参考范围的Ig A、Ig M结果差异具有显著性(P<0 .0 5 ) ,相关系数分别为r=0 .994和r=0 .989。当Ig G大于5 5 g/ L时,出现假性低值。结论 两法在一定浓度高值范围相关,定量结果间存在差异。速率散射比浊测定法具有灵敏度高,自动识别抗原过量所致的钩状现象并进行自动稀释处理、准确定量的优点。透射比浊在全自动生化分析仪上应用不具备前者的功能,因此需对单克隆Ig异高值病例初诊加以抗原过量的防范,可采用电泳或蛋白定量过筛,避免单克隆Ig异高值漏检。
Objective To investigate the access to assay of the abnormally high level serum mono-clonal immunoglobin(Ig) with immunoturbidimetry. Methods Using 5 points calibration,30 samples of abnormally high level serum IgG were measured with immunoturbidimetry on both Hitachi 7170A system and Beckmen protein analytical system. Results The levels of IgG in mono-clonal immunoglobin disease were significantly different by using the two methods. The correlation factors to IgG, lgA and IgM were 0.966,0.994 and 0.989 respectively in definite concentration. When IgG was over 55g/L, false negative case was found. Conclusion The nephelometry of protein analytical system is more sensitive and can identify access antigen(Ag) by auto- procedure. Transmission-turbidimetry is a practical analysis system used in clinic laboratory, but we must beware of the presence of false negative case. Thus, a screening test showing high antigen quantity by protein- electrophoresis or serum total protein analysis is recommended when mono-clonal immunoglobin disease is suspected.
出处
《西部医学》
2005年第3期266-267,共2页
Medical Journal of West China
