摘要
为了给中药治疗骨折愈合提供动物实验依据, 将32只6月龄新罗曼母鸡, 体重1 75kg左右, 随机分为数目相等的两组, 中药组和对照组, 无菌手术条件下制备右肱骨中部一致性骨折模型, 骨折后中药组于饲料中添加1 5%复方中药(当归、川芎、桃仁、红花、血竭、土元、黄芪、川续断、骨碎补、乳香、没药、丹参、自然铜、大黄等量混合), 对照组常规喂料。骨折后3d、5d、10d和15d心脏采血检测血清碱性磷酸酶, 以骨折局部3d、5d、10d和15d的组织材料制作组织切片, 利用VonKossa氏法进行钙质染色检测骨痂钙盐沉积情况。结果: 中药组血清碱性磷酸酶活性骨折后6d、10d明显高于对照组, 差异极显著(P<0 01), 切片显示中药组骨折后10d和15d中药组钙盐沉积情况优于对照组。结论: 中药对鸡实验性骨折愈合有明显促进作用; 中药对鸡血清碱性磷酸酶活性, 骨痂钙盐沉积有促进作用。
To clarify the effects of Chinese herbal medicine on fracture healing, we conducted the studies. 32 of 6 month old New Roman hens, weight about 1.75 kg, were randomly divided into two groups, Chinese herbal medicine group(n=16) and control group(n=16) and were established right humerus fracture model. Add 1.5% Chinese herbal medicine (Angelica sinensis , Prunus persica, Drynaria fortunei, et al. ) to the food of Chinese Herbal medicine group animals, while control group animals were fed as normal. Get blood from the heart of hens on 3 d,6 d,10 d and 15 d after fracture and measure their serum ALP. The calcium of callus on 3 d,6 d,10 d and 15 d after fracture were examined by Von Kossa method. The values of Chinese herbal medicine group serum ALP were higher significantly than control group at 6 d,10 d (p<0.01). Calcification of Chinese herbal medicine group was better than that of control group. Chinese herbal medicine played a significantly role in experimental chicken fracture healing; it also enhanced serum ALP and calcium of callus.
出处
《山西农业大学学报（自然科学版）》
CAS
2005年第2期119-122,共4页
Journal of Shanxi Agricultural University(Natural Science Edition)
关键词
骨折愈合
中药
鸡
血清ALP
钙沉积
Chickens
Fracture healing
Chinese herbal medicine
Serum ALP
Calcification
