摘要
目的 探讨生存素(surviving)在正常子宫内膜、增生子宫内膜以及子宫内膜癌中的表达;并探讨生存素与子宫内膜癌的临床分期、病理级别及浸润子宫肌层深度的关系。方法 取正常周期、单纯增生、不典型增生子宫内膜各2 0例;萎缩性子宫内膜10例以及子宫内膜癌2 0例,应用人生存素多克隆抗体,采用免疫组织化学SSCP法,观察其表达情况。结果 生存素在2 0例分泌期子宫内膜中全部表达,细胞浆呈均匀染色;2 0例增殖期子宫内膜,6 0 %呈细胞浆均匀染色染色;10例萎缩型子宫内膜均不表达生存素;2 0例单纯增生子宫内膜全部表达生存素,细胞浆均匀染色;2 0例非典型增生子宫内膜全部表达生存素,但间质细胞表达弱于腺上皮细胞;30例子宫内膜癌细胞10 0 %表达生存素,特点是10 0 %浆表达,间质细胞表达明显弱于腺上皮细胞,同时4 3 33%核表达,且核表达率与临床期别、病理级别、侵犯基层深度有密切关系,统计学差异显著结论 生存素不是子宫内膜癌特有的标志蛋白,但可能是预后判断和监测治疗的指标。
Objective To investigate the expression and location of surviving in normal cycle endometrium and endometrial carcinomal.Methods We analyzed serial frozen section for survivin expression by immunhistochemistry.Restults survivin was detected in all 20 cases secretory phase,was detected in 12 out of 20 cases prolieferation phase,was not detected in the all 10 cases atrophic endometrial.Staining is localized to the cytoplasm.Survivin expressed in the all 20 cases hyperplasia,staining was localized in the cytoplasm,the characterize is that the staining in the 60% stromal cells are weaker than in the glandular epithelial cells.In the 30 cases endometrial carcinoma,there was abundant surviving immunoreactivity in the 60% nucleus and 100% cytoplasm,the staining in the glandular epithelial cells is stronger than in the stromal cells,surviving expression was significantly associated with clinical stage,histological,grade,the presence of invasion to ≥1/2 myometrium.Conclusions survivin is not the marker protein for the endometrial carcinoma,but it is a defining prognostic and decided the curative effect marker.
出处
《中国妇产科临床杂志》
2005年第3期189-191,222,共4页
Chinese Journal of Clinical Obstetrics and Gynecology
