摘要
目的 探讨卵巢癌抗独特型抗体6B11表位多肽与诱导免疫应答之间的关系。方法 采用化学合成的方法,合成抗独特型抗体6B11轻重链可变区的六条CDR区,分别以三种不同浓度免疫BALB/c小鼠。采用ELISA的方法检测免疫鼠血清,进行体内免疫学功能研究。结果 6B11抗独特型抗体的六条CDR肽分别加入佐剂免疫小鼠后,其中有两条肽可以诱导出Ab3,分别为重链的CDR2区和轻链的CDR2区。结论 这两条肽作为后选肽有可能是6B11抗独特型抗体特异性的CTL表位。
Objective To explore the relationship between the immune response induced by 6B11 anti-idiotypic antibody and its epitope peptides. Methods Six CDR peptides of 6B11 anti-idiotypic antibody were synthesized. BALB/c mice were immunized repeatedly by different doses of six peptides with adjuvant, respectively . Indirect ELISA was used for analyzing the serum characterization of anti-anti-idiotypic antibody (Ab3). Results In all six peptides only two , VLCDR2 and VHCDR2 peptides could induce the specific humoral immune response and the Ab3 could be detected in immunized BALB/c mice serums. Conclusions The two candidate peptides were perhaps CTL epitope peptides.
出处
《中国妇产科临床杂志》
2005年第3期204-207,共4页
Chinese Journal of Clinical Obstetrics and Gynecology
基金
首都医学发展基金 (2 0 0 3 -2 0 0 3 )
国家自然科学基金 (3 0 47195 9)资助
