摘要
本文建立了测定人血清及尿样中索他洛尔（ｓｏｔａｌｏｌ）的反相ＨＰＬＣ方法。索他洛尔及内标氨酰心安（ａｔｅｎｏｌｏｌ）的色谱分析采用氰基分析柱及保护柱，流动相为乙腈：甲醇：１０％磷酸：１０％正丁胺（７５：２５：０．２：０．１，ｖ／ｖ）。流速为１．８ｍｌ／ｍｉｎ。血样及碱化尿样用溶剂萃取（氯仿：异丙醇４：１，ｖ／ｖ），采用荧光检测器检测，其激发和发射波长分别为２２０ｎｍ及３１３ｎｍ。实验结果表明：索他洛尔与内标的分离良好，其保留时间为３．５ｍｉｎ和５．０ｍｉｎ。在血样及尿样中的萃取的绝对回收率：索他洛尔＞９０％；内标＞８０％。线性范围：血样为０－５ｍｇ／ｌ（０．９９９９）；尿样为０－５０ｍｇ／ｌ（０．９９９８）。日内及日间差异均小于１０％。最低检测下限为０．０１３ｍｇ／ｌ。本方法适用于索他洛尔的药代动力学研究。
A simple and sensitive HPLC method for the measurement of sotalol in human serum and urine has been developed.Seperation was obtained using a NovPak CN column.The mobile phase is consisted of a mixture of acetonitrile-methanol-10% phosphoric acid-10% buthylamine(75:25:0.2:0.1,v/v).Sotalol and internal standard.atenolol.were extracted from serum or alkalinized urine into chloroform-2-propanol(4:1,v/v).The retention times of sotalol and I.S.were 3.5 min and 5.0 min respectively.Within-day and day to day coefficients of variation were less than 10% in serum and in urine.Calibration was linear from 0-5.0 mg/1 in serum(r=0.9999)and from 0-50mg/1 in urine(r=0.9998).The limit of detection was 0.013 mg/1 with spectrofluorimetric detector at 220 nm excitation and 313 nm emmision wavelength.This method has been demonstrated to be suitable for pharmacokinetic study comparing with other report.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
1994年第4期215-219,共5页
The Chinese Journal of Clinical Pharmacology
