摘要
通过研究ＦＫ５０６在体外对人淋巴细胞趋化反应的影响。发现在ＦＫ５０６的浓度为０．５ｎｇ／ｍｌ时，对蛋－亮－脯氨酸甲酯多肽（ＦＭＬｐ－ＭＥ）、酵母多糖激活血清（ＺＡＳ）和趋化性胆汁（ｃｈｅｍｏｔａｃｔｉｃｂｉｌｅ，ＣＢ）引起的淋巴细胞趋化反应的抑制率分别为４７．３％、３２．２％和４８．９％；对经ＰｈｏｒｂｏｌＭｙｒｉｓｔａｔｅＡｃｅｔａｔｅ（ＴＰＡ）激活的淋巴细胞，ＦＫ５０６的抑制作用更强，抑制率分别达７１．２％、５５．４％和６９．６％。ＦＫ５０６也抑制ＴＰＡ激活的淋巴细胞产生趋化因子。比较经不同处理的淋巴细胞培养上清液引起的白细胞趋化反应，发现ＴＰＡ组与ＴＰＡ＋ＦＫ５０６组间存在显著性差异（淋巴细胞趋化反应Ｐ＜０．００１、单核吞噬细胞Ｐ＜０．０００５、中性粒细胞Ｐ＜０．０００５），而对未激活的淋巴细胞产生趋化因子无影响。可见对淋巴细胞趋化反应的抑制可能是ＦＫ５０６作用的一种方式。
AbstractAdams D. Liver Unit Queen
Elizabeth Hospital in Birmingham. England We have studies the effect
of FK506 on the chemotaxis of human lymphocyte in vitro.
Theinhibitory activities of FK506 in concentration of 0.5ng/ml on the
lymphocyte chemtaxis stimu-lated by FMLp-ME,ZAS or CB were
71.2%,55.4%and.69.5%respecticvely,It showed adose dependent pattern
of inhibitory activity of FK506,i.e. the higher concentration of
FK506,the stronger inhibition of chemotaxis of lymphocytes.In
comparison with above data,markedhigh inhibitory activities were
observed in the lymphocytes prestimulated by TPA with the in-hibitory
rate of 71.2%,55,4%and 69.6% separately,A significant difference of
chemotaxis forvarious cell type was observed in the supernatants of
lymphocyte culture activated by TPA aloneor TPA with FK506(lymphocyte
P<0.0O1;moncyte-macrophage P<0.0005; Neutrophil P<0.0005 ).No
difference of chemotaxis in supernatants of unstimulated lymphocytes
was re-vealed between FK506 and control.It is suggested that the
therapeutic mechanism of FK506might be by means of inhibition of the
chemotaxis of lymphocyte.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1994年第1期28-31,共4页
Chinese Journal of Immunology
