摘要
采用酶标抗体竞争结合试验对６株抗ＩＬ－８单克隆抗体识别的表位进行了鉴定。按识别表位的不同可将６株单克隆抗体分为两组，一组包括２Ｇ２、４Ｃ３和４Ｅ１，识别相同或相近的表位；另一组包括４Ｄ７、５Ｃ９和５Ａ４，识别相同或相近的表位。在表位鉴定的基础上，选择识别不同表位的单克隆抗体用于建立双单克隆抗体夹心法ＥＬＩＳＡ检测ＩＬ－８，其中以４Ｄ７为包被抗体，４Ｃ３为酶标抗体的组合可获得最高的敏感性，对单核细胞源性ｒｈＩＬ－８（ＭＩＬ－８）及内皮细胞源性ｒｈＩＬ－８（ＥＩＬ－８）检测的敏感性可分别达到１５６ｐｇ／ｍｌ及３１２ｐｇ／ｍｌ。
pitopes recognized by 6 monoclonal antibodies to IL-8 were characterized by HRP labelledantibody competitive binding assay.It was found that these 6 monoclonal antibodies can be divid-ed into two groups according to the epitopes they recognized.2G2,4C3 and 4E1 belong to a sin-gle group while another group include 4D7,5A4 ang 5C9.On this basis,a sandwich ELISA wasestablished using 4D7 as coating antibody and HRP labelled 4C3 as second antibody with its de-tection limit about l50pg/ml.
出处
《中国免疫学杂志》
CSCD
北大核心
1994年第3期131-133,共3页
Chinese Journal of Immunology
