摘要
经ＩＬ－２基因转移瘤苗治疗后荷瘤小鼠肺转移结节明显减少、存活期明显延长，与ＩＬ－１或低剂量Ｃｙ合用后，可使荷瘤小鼠的肺转移结节更少，存活期更长，特别是当ＩＬ－２基因转移的瘤苗、ＩＬ－１、低剂量Ｃｙ三者合用时抗肿瘤转移效果最强。对瘤苗治疗后荷瘤小鼠体内免疫功能的研究表明，小鼠脾脏ＣＴＬ活性、ＮＫ活性、ＩＬ－２导的ＬＡＫ活性均显著增强，脾脏细胞分泌ＩＬ－２和ＴＮＦ水平也显著升高，当与ＩＬ－１、低剂量Ｃｙ合用时，上述抗肿瘤免疫功能升高得更加明显，可见ＩＬ－２基因转移的瘤苗能通过有效地激活体内抗肿瘤免疫功能而具有显著的抗肿瘤转移效果，将其与ＩＬ－１或低剂量Ｃｙ联合应用时抗肿瘤效果更佳，当三者同时合用时抗肿瘤效果最佳。
After treatment with IL-2 gene-transfected vaccine alone,the pulmonary metastases werereduced and the survival time of tumor-bearing mice was prolonged, The anti-metastatic effectwas better when the vaccine was used with IL-1 or Iow-dose Cy. The best therapeutic effectwas achieved when IL-2 gene-transfected vaccine, IL-1,Iow-dose Cy were all used in the sametime. The cytotoxicity of murine splenic CTL、NK cells、IL-2 induced LAK cells,and the levelof IL-2,TNF secreted by splenocytes increased significantly after tumor-bearing mice weretreated with the vaccine. The above antitumor immune functions were augmented more signifi-cantly when IL-1、 low-dose Cy were also used. These results demonstrated that the vaccinetransfected with IL-2 gene had effective anti-metastatic effect through the activation of the in vi-vo antitumor immunity. When it was used with IL-1 and/or Iow-dose Cy,the therapeutic effect was better.
出处
《中国免疫学杂志》
CSCD
北大核心
1994年第5期289-293,共5页
Chinese Journal of Immunology
基金
上海市科技启明星计划和国家自然科学基金
关键词
白细胞介素2
肿瘤
疫苗
环磷酰胺
Interleukin- 2 Gene therapy Cancer vaccines Interleukin-1 Cy-clophosphamide
