摘要
目的建立液-质联用(HPLC-MS)测定人血浆中地塞米松浓度的方法。方法采用Agilent Zorbax Extend C18色谱柱(2.1 mm×150 mm,5 μm),以0.4%醋酸铵溶液-乙腈(62:38)为流动相,流速0.25 mL·min-1,在血浆中加入内标醋酸泼尼松,固相萃取后采用HPLC-MS(ESI源)测定地塞米松的血浆浓度。结果地塞米松在人血浆中0.02-4 mg·L-1内线性良好,最低检测限为0.02 mg·L-1,绝对回收率>95%,相对回收率在98.84%-102.80%之间,日内及日间RSD均小于3%。结论本方法能满足人血浆中地塞米松浓度的测定。
OBJECTIVE: To develop and validate a HPLC-MS method for determining dexamethasone in human plasma. METHODS: The separation was performed on a column of Zorbax Extend C18 (2.1 mm × 150 mm, 5 μm). The mobile phase consisted of 0.4% ammonium acetate solution-acetonitrile (62:38). Using solid phase extraction, dexamethasone in human plasma was measured by HPLC-MS with ESI. The internal standard was prednisone acetate. RESULTS: The standard curve was linear over the range of 0.02-4 mg·L-1. The lowest dexamethasone concentration that gave a reproducible integration was 0.02 mg·L-1. The absolute recovery was more than 95%, the relative recovery was among the range of 98.84%-102.80%. The RSDs of intra-day and inter-day validation were less than 3% CONCLUSION: HPLC-MS may be used for the determination of dexamethasone in human plasma.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2005年第9期705-707,共3页
Chinese Pharmaceutical Journal
关键词
地塞米松
高效液相色谱-质谱
血药浓度
Acetonitrile
High performance liquid chromatography
Mass spectrometry
Plasmas
Solutions
