摘要
用抗牛型结核杆菌单克隆抗体（ＭｃＡｂ）１Ａ３和抗牛型结核杆菌免疫血清经酶联免疫吸附试验（ＥＬＩＳＡ）对１９种分支杆菌参考株进行检测，证明了抗牛型结核杆菌ＭｃＡｂ１Ａ３的特异性和抗牛型结核杆菌免疫血清（ＩＭＳ）的相对非特异性。用ＭｃＡｂ１Ａ３对３６株分支杆菌临床分离株的早期培养物（培养３周）进行菌型鉴定，所需菌量最少仅２×１０７，即可鉴定出临床分离株中的牛型结核杆菌，敏感性和特异性均为１００％。此方法较常规微生物鉴定方法简单、省时，是一种有发展前途的菌型鉴定方法。
A simple enzyme-linked immunosorbent assay(ELISA) using monoclonal antibody(McAb) 1A3 and immunoserum(IMS)against mycobacterium bovis for the testing 19 mycobacterium reference strains,proved the specificity of McAb 1A3 and unspecificity of IMS against mycobacterium bovis.In the ELISA only 2 ×107 bacteria derived from early culture(3 weeks) were needed for McAb 1A3.The results of identification of 36 mycobacterium field isolates by microbiological means and by ELISA were compared; For the M.bovis,the sensitivity and specificity were both 100%.The ELISA described here proved to be useful in both well and modestly equipped laboratories and may replace the microbiological methed of identification of M. bovis.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1994年第2期38-40,共3页
Chinese Journal of Zoonoses
