摘要
本文利用ＤＨＢＶ阳性血清感染１日龄雏鸭，感染后２、４、８周以血清斑点杂交和Ｄｏｔ－ＥＩＡ检测鸭血清中的ＤＨＢＶ－ＤＮＡ和ＤＨＢｓＡｇ，其阳性率分别为５６．３％、６５．６％、７４．２％和５３．１％、７１．９％、７０．９％。进一步采用原位杂交和免疫组化对鸭肝组织内ＤＨＢＶ－ＤＮＡ和ＤＨＢｓＡｇ进行检测，结果表明ＤＨＢＶ－ＤＮＡ主要存在于细胞浆中，而ＤＨＢｓＡｇ主要分布于细胞浆及细胞膜。对肝组织的病理检查及胶原纤维特殊检测发现，ＤＨＢＶ阳性组与阴性组无明显差别。
ne-day-old ducklings were infected by DHBV-positive serum.2、4、8 weeks later,serum DHBV-DNA and DHBsAg of all ducks were detected and the positive rate of them were 56.3%、65.6%、74.2% and 53.1%、71.9%、70.9% respectively. DHBV-DNA and DHBsAg in the liver tissue were further detected by situs hybridization and immuno-histochemistry respectively.The results indicated that DHBV-DNA mainly distributed in cytoplasm and DHBsAg predominantly distributed not only in cytoplasm but also in cell membrane.Meanwhile ,with the observation of pathology and the special detection of collagenous fibers in liver tissue,the results showed no apparent differences between DHBV positive and negative ducks.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1994年第6期2-5,共4页
Chinese Journal of Zoonoses
