摘要
以小麦DH群体(旱选10号×鲁麦14)为研究材料,采用复合区间作图法,对小麦幼苗在水分胁迫及非胁迫条件下的水分利用效率(WUE)及其相关性状的QTL进行定位,并对比分析QTL的加性效应。两种水分条件下共检测到14个具显著加性效应的QTL,分布在2A、3A、4A、5A、6A、7A、1B、3B、3D染色体上,可解释表型变异的范围在6.36%-19.73%。其中,非胁迫(对照)条件下检测到10个QTL,包括2个单株WUE的QTL,5个地上部WUE的QTL,1个根系WUE的QTL及2个总耗水量的QTL;水分胁迫条件下上述性状各检测到1个QTL。对于同一性状没有检测到在两种水分条件下均位于同一标记区间的QTL,表明不同水分环境条件下同一性状的QTL表达模式是不同的。论文也讨论了可能用于标记辅助选择的QTL及其分子标记。
A doubled haploid (DH) population of 150 lines, derived from a cross between two Chinese com mon wheat varieties Hanxuan10 and Lumail4 was used in this study to map quantitative trait loci ( QTL) for water use efficiency (WUE) and related traits at seedling stage under water stress and control conditions, respectively. Mixed-model-based composite interval mapping analysis revealed that a total of 14 QTL of additive effect for the target traits were mapped on chromosomes 2A, 3A, 4A, 5A, 6A, 7A, 1B, 3B and 3D. The contribution range of these QTL to phenotypic variation was from 6.36% to 19.73% . Of these QTL, 10 were de tected under control condition: two for WUE, five for up-ground water use efficiency (UGWUE), one for down-ground water use efficiency (DGWUE) , and two for water consumed (WC); under water stress condition, one QTL was detected for each trait, respectively. There was no QTL regulating the same given trait to be mapped on the same marker interval under both conditions, implying that there were different QTL expression patterns under different water conditions. Some QTL may be employed to perform marker assisted selection in breeding.
出处
《植物遗传资源学报》
CAS
CSCD
2005年第1期20-25,共6页
Journal of Plant Genetic Resources
基金
国家863计划(2002AA224011)国家973计划(2003CB114301)资助项目
