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免疫印迹法分析AFP糖链及其临床意义

Immune Blotting System for the Detection of AFP Variants and Its Clinical Significance
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摘要 本文采用免疫印迹法分析扁豆凝集素与AFP糖链的结合能力,鉴别诊断肝细胞癌。对32例肝细胞癌、14例慢性肝炎、肝硬化患者的AFP逆行了分析,以扁豆凝集素结合型AFP部分大于总量的25%作为诊断肝细胞癌的标准,其中阳性率为100%(32/32),正确率为73.9%(34/46)。 Immune blotting system was developed for the detection of AFP variants which was separated by LCA Affinity electrophoresis in agarose gels. The sera AFP from 32 primary hepatocellular carcinoma (PHC) patients and 14 patients with benign liver diseases were studied.The results showed that the AFP-R-LcA of PHC were higher than the benign liver diseases. The positivity was 100%(32/32) in PHC patients.
出处 《中国生化药物杂志》 CAS CSCD 1994年第4期238-241,共4页 Chinese Journal of Biochemical Pharmaceutics
关键词 肝肿瘤 免疫印迹 甲胎蛋白 AFP variants Immune blotting PHC
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