摘要
探讨了外生菌根菌耳环乳牛肝（Ｓｕｉｌｌｕｓｇｒｅｖｉｌｌｅｉ）的原生质体制备及再生条件。用ＭＹＧ培养基培养菌丝７天、宋用１．５％Ｌｙｗａｌｌｚｙｍｅ，以０．６ｍｏｌ甘露醇为稳渗剂，在酶解液ｐＮ值５．５，温度３１℃，酶解４ｈｒ的条件下，每０．１ｇ湿菌丝的原生质体产量这６×１０￣６／ｍｌ。用ＳＯ培养基培养菌丝能提高原生质体产量，但再生率下降。综合产量和再生率考虑以ＭＹＧ培养菌丝效果较好。
Conditions for preparingthe protoplasts from mycelia of ectomycorrhizal fungus SMillus grevillei were examined.The highest production of protoplasts(6 ×10￣6/ml per 0.1g fresh mycelia) was abtained with the enzyme mixture of pH 5.5 containing 1.5% Lywallzyme,0.6mnol/L mannitol as osmotic stabilizer,and incubated at 31℃, from 7-day age mycelia cultured in MYG medium. UsingSO miedium instead of MYG doubled the protoplast productlon, but markedly decreased the plating efficiency. It is concl-uded that MYG is a better medium than SO for the preparation of protoplasts from Suillus previlllei.
出处
《中国食用菌》
1994年第2期24-26,共3页
Edible Fungi of China
基金
国家教委资助基金
关键词
牛肝菌
原生质体
制备
细胞工程
Ectomycorrhizal fungi
Protopiast prepartion
Cytological engi-neering
