摘要
目的通过研究活血药的提取物丹参素和川芎嗪对血管紧张素Ⅱ(AngⅡ)致心肌肥大及相关基因的影响,探讨其抑制心肌肥大的作用机理。方法以心钠素(ANP)和肌动蛋白- β(β- actin)基因表达为指标,采用一步法,应用TRIzolReagent提取心肌细胞总RNA ,然后用RT PCR方法测定其ANP、β- actin的mRNA表达。结果分子生物学研究表明,AngⅡ可显著增加心肌细胞ANPmRNA的表达(P <0 . 0 1) ,而Losartan可明显抑制AngⅡ所诱导的ANPmRNA的表达(P <0 . 0 1) ,丹参素和川芎嗪也可减少ANPmRNA的表达(P <0 . 0 5 ) ;AngⅡ同时也增加心肌细胞-βactinmRNA的表达,而Losartan、丹参素和川芎嗪可显著抑制其表达(P <0 .0 5 )。结论活血药的有效组分丹参素和川芎嗪可抑制AngⅡ对心肌细胞ANP和β-actin基因表达的增加,具有防止心肌细胞肥大作用,从而防治心脏肥厚。
ObjectiveTo study the effect of Danshensu (DSS) and Ligustrazine (TMZ), the extracts of Chinese herbs for promoting blood circulation, on angiotensin Ⅱ(Ang Ⅱ) induced myocardial hypertrophy and its related genes, and to explore the mechanisms of inhibitory effect. MethodsAdopting one-step method, the total RNA of myocardial cells was extracted by TRIzol reagent. Then the expression of ANP and β-actin mRNA, as symbol of myocardial cells, were detected by RT-PCR. ResultsMolecular biological research showed that AngⅡ could significantly increase the expression of ANP mRNA in myocardial cells (P<0.01), which could be significantly inhibited by Losartan (P<0.01), both DSS and TMZ had the inhibitory effect (P<0.05). AngⅡ could increase β-actin mRNA expression in myocardial cells simultaneously, Losartan, DSS and TMZ could also significantly inhibit it (P<0.05). ConclusionThe effective ingredients of Chinese herbs for promoting blood circulation, DSS and TMZ, have the effect of inhibiting the hyper-expression of ANP and β-actin induced by AngⅡ, and preventing myocardial hypertrophy, therefore, it could be used to prevent and treat cardiomegaly.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2005年第4期342-344,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家中医药管理局重点课题 (No .972 0 69)
