摘要
目的观察蜂胶体外抗阴道毛滴虫的效果。方法阴道毛滴虫体外无菌培养后,加入蜂胶溶液使其浓度为2.4mg/ ml,同时设置溶剂对照组和正常培养空白对照组,分别于0h、6h、12h和24h观察各组虫数、活率并计算活虫数。结果培养0 h、6h、12h、24h不同时间蜂胶组的虫数(万/ml)分别为50.50±2.92、16.25±2.40、12.88±0.85、11.13±1.31;活虫数(万/ml) 46.20±2.91、7.04±1.75、2.87±0.69、1.24±0.59;活率(%)91.50±3.11、43.00±6.83、22.25±5.32、11.50±5.74。空白对照组的虫数分别为43.25±8.29、39.00±13.22、55.50±18.01、81.88±22.63;活虫数37.68±7.56、36.77±12.28、50.09±17.19、79.61±22.09;活率87.00±0.82、94.50±3.51、90.00±4.32、97.25±0.96。蜂胶组与溶剂对照组以及空白对照组比较差异有显著性。结论蜂胶具有明显的体外抗阴道毛滴虫效果,培养液蜂胶浓度为2.40mg/ml时,随培养时间延长,滴虫不断减少,活率不断降低。
Objective To investigate the antitrichomonas effect of propolis from Henan Province of China on Trichomonas vaginalis after being cultured for different hours in vitro. Methods The culture of T. vaginalis were divided into three groups: the propolis group, the solvent group and the control group. The concentration of propolis in the medium of propolis group was 2.4 mg/ml, equivalent amount of solvent was added to the solvent group, and the control group was normally cultured. The number of the parasite, the number of the survivals and the survival rate at 0, 6, 12 and 24 hours were observed and recorded respectively after being cultured. Results After being cultured for 6, 12 and 24 hours, the number of the survivals(10~4 /ml) in the propolis group was: 7.04±1.75, 2.87±0.69, 1.24±0.59; the survival rate (%) was: 43.00±6.83, 22.25±5.32, 11.50±5.74. In the control group, the number of the survivals: 36.77±12.28, 50.09±17.19, 79.61±22.09; the survival rate: 94.50±3.51, 90.00±4.32, 97.25±0.96 respectively. Significant difference was found between the propolis group and the others. Conclusion Propolis collected from Henan Province possesses obvious antitrichomonas activity in vitro, the number of individuals would significantly decrease and the survival rate would strongly decline in the medium with propolis concentration at 2.40 mg/ml.
出处
《中国寄生虫病防治杂志》
CSCD
2005年第2期116-119,共4页
Chinese Journal of Parasitic Disease Control
基金
河南省教育厅自然科学基础研究资助项目(No.2003310120)。~~
关键词
蜂胶
阴道毛滴虫
抗滴虫
Trichomonas vaginalis
propolis
antitrichomonas
