摘要
目的 探讨输血后不同时间、不同输血量供者STRs基因型对受者STRs基因型的影响。方法 采集3例患者输血前以及输血后4 h、8h、12 h的血液和供者血液,EDTA抗凝,用Chelex- 10 0法提取DNA,选D1S5 4 9、D18S86 5、D3S175 4、D12 S391、D12 S375、D6 S4 77等6个STRs基因座进行PCR扩增,扩增产物用聚丙烯酰胺凝胶垂直板电泳,银染显色分型。结果 6个STRs基因座的检测结果显示受者输血前及输血后12 h内STRs基因型一致。结论 输血量不大于12 0 0 ml的受者输血后12 h内STRs基因型未受供者血液STRs基因型的影响。
Objective To investigate whether any change occurred in recipients' blood collected at different times after transfusion with different quantity of blood. Methods Three patients were transfused with 400 ml, 800 ml and 1200 ml blood separately. The blood samples were collected from the recipients before transfusion and at 4 h, 8 h, 12 h after transfusion, and from the donors. DNA were extracted by Chelex-100 method and were amplified by the polymerase chain reaction (PCR). Six loci of D1S549,D18S865,D3S1754,D12S391,D12S375,D6S477 were selected. The PCR products were analyzed by polyacrylamide gel (PAG) vertical electrophoresis and silver staining. Results The investigations on the six STR loci revealed that the patients' STR genotypes remained unchanged within 12 h after blood transfusion. Conclusion The STR genotypes of the donors would have no influence on the STR genotypes of the patients within 12 h after transfusion providing the volume of blood transfused is less than 1200 ml.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2005年第3期359-361,414,共4页
Journal of Sichuan University(Medical Sciences)
关键词
输血
STR基因座
聚合酶链反应
Blood transfusion STR locus Polymerase chain reaction
