摘要
目的:应用cDNA基因芯片研究筛选人胃腺癌相关差异表达基因,探讨胃癌发生、发展的分子机制.方法:运用含18000个基因克隆的cDNA膜基因芯片,对6对胃腺癌及正常胃组织标本分别进行杂交检测并对比分析.结果:6对人胃腺癌与相对应的胃正常组织相比较,筛选出在3～6对标本中有2倍以上改变的基因克隆共有103条(1条在所有标本中均差异明显),其中胃癌组织表达上调基因56条,胃癌组织表达下调基因47条.结论:人胃腺癌癌变是一个多基因参与的过程,基因芯片技术是一种快速有效的筛选人胃腺癌差异表达基因的方法.
OBJECTIVE:Despite the increase in our knowledge of oncogenes and tumor suppressor genes associated with gastric cancer over the past decades, the mechanism of the process of multistage carcinogenesis is still unknown for gastric cancer. The study was designed to investigate the changed gene expression patterns in the gastric carcinogenesis and screening the gastric cancer related genes would provide a credible key to improve the dignosis and therapy of gastric cancer.METHODS:Differently expressed genes of gastric adenocarcinoma in 6 cases were investigated by using high density cDNA microarray representing 18 000 cDNA clones.RESULTS:The genes whose ratios of T/N (gastric adenocarcinoma/normal gastric mucosa) were higher than 2.0 or lower than 0.5 were screened out after hybridization.The expression level of 103 genes differed between gastric adenocarcinoma and normal mucosa in 3 or more samples (one gene differed in all samples ). There were 47 downregulated and 56 upregulared genes in gastric adenocarcinoma.CONCLUSIONS:cDNA microarray technique is effective in screening the differentially expressed genes between gastric adenocarcinoma and normal mucosa.The present study represents a global view of gene expression of gastric adenocarcinoma and provides important clues for further study of gastric adenocarcinoma related genes.
出处
《肿瘤防治杂志》
2005年第8期571-575,共5页
China Journal of Cancer Prevention and Treatment
基金
全军"十五"医学科研基金重点课题(01Z035)
关键词
胃肿瘤/遗传学
腺癌/遗传学
基因表达
DNA
互补
克隆
分子
杂交
遗传
stomach neoplasms/genetics
adenocarcinoma/genetics
gene expression
DNA,complementary
cloning,molecular
hybridization,genetic
