摘要
目的建立能客观、真实地反映人血管瘤的临床发展过程的稳定的血管瘤裸鼠移植模型,动态观察其生物学特性的变化。方法以裸小鼠为载体,选取1例2月龄婴儿增生期毛细血管瘤,将瘤体组织转种于裸小鼠皮下,动态观察瘤体生长情况及组织学变化,检测不同时期移植血管瘤糖皮质激素受体、血管内皮细胞生长因子、Ki67的表达。结果血管瘤组织移植后3～4周内,瘤体基本上无明显变化,40d后,大多数瘤体开始生长,60d左右达到最大,90d时可见部分瘤体颜色变浅,质地较前变硬,90～120d时部分瘤体仅有少许残留组织。糖皮质激素受体、血管内皮细胞生长因子、Ki67在增生期移植血管瘤中高表达,而在退化期移植血管瘤中表达极弱。糖皮质激素受体与Ki67、血管内皮细胞生长因子的表达正相关,Ki67与血管内皮细胞生长因子表达正相关。结论将人体血管瘤皮下部分移植于裸鼠所建立的人血管瘤动物模型,能较真实地反映人血管瘤的发展过程,是研究血管瘤的较理想的平台。糖皮质激素受体、血管内皮细胞生长因子与移植血管瘤的演变过程有着密切的关系。
Objective To establish a reliable model of human hemangioma in nude mice and to observe the biological properties of xenografted human hemangioma. Methods The specimen of proliferating hemangioma from one 2-month male infant was obtained by surgery. The tissue was cut into small pieces 5 mm×4 mm×3 mm in size and grafted into nude mice subcutaneously. Gross observation and histological examination were carried out at 30, 45, 52, 59, 66, 80, 90, and 120 day after graft, respectively. Immunohistochemistry and image analysis were employed to detect the expression of glucocorticoid receptor (GR), vascular endothelial growth factor (VEGF), and Ki-67 at different time points. Results Within 3～4 weeks after graft, no dramatically enlargement in size was observed. Fortieth day after graft, most grafts were observed to grow with obvious neovascular network formation surrounding the grafts. The volume of graft hemangioma achieved the maximum at about 60 day after graft. Ninetieth day after graft, some grafts turned lighter and harden, with decrease in volume. Living grafts held 83.3% of all grafts. Some residuum would be recognized at 120 day after transplantation. High positive staining of GR, VEGF, Ki-67 were obtained in grafts in proliferative phase and located in the cytoplasm of plump endothelial cells, while it was hardly recognizable in grafts in involuting or involuted phases. Coefficient correlations of GR to Ki-67 and VEGF were 0.766 (P< 0.01) and 0.643 (P< 0.01), respectively. Coefficient correlation of VEGF to Ki-67 was 0.763 (P< 0.01). Conclusions The patterns of human hemangioma can be remained in this animal model. And the course of change in graft hemanioma is related to the expressions of GR, VEGF and Ki-67.
出处
《中华小儿外科杂志》
CSCD
北大核心
2005年第5期264-267,共4页
Chinese Journal of Pediatric Surgery
基金
教育部博士点基金资助(批准号:200220610068)
四川省科技厅基金资助(批准号:02sy029115)
