摘要
目的 将BRCA1真核表达质粒转染人乳腺癌MCF 7细胞中,观察外源性BRCA1基因转染MCF 7细胞后对hTERT蛋白表达的抑制作用及对细胞增殖的影响。方法 应用脂质体法将BRCA1真核表达质粒转染至乳腺癌MCF 7细胞中,Westernblot检测转染细胞中hTERT蛋白表达的变化。MTT比色试验观察BRCA1真核表达质粒转染后MCF 7细胞增殖活性的变化。结果 外源性BRCA1真核表达质粒组MCF 7细胞较未转染组MCF 7细胞hTERT蛋白表达明显减弱。BRCA1基因转染后的MCF 7细胞的增殖活性明显降低。结论 外源性BRCA1基因的表达可以抑制人乳腺癌MCF 7细胞中hTERT基因的表达,并抑制MCF 7细胞的增殖能力。BRCA1基因的表达异常可能在乳腺肿瘤发生发展机制中具有重要的作用。
Objective To transfect the recombinant eukaryotic expression vector containing BRCA1 cDNA into human breast cancer cell line MCF-7 and explore the effects of exogenous BRCA1 gene on the expression of hTERT protein in MCF-7 cell line. Methods pCDNA3.1-BRCA1 plasmid was transfected into MCF-7 cell line by lipofectin reagent. Expression of hTERT protein was determined by Western blotting. The influence of exogenous BRCA1 gene on proliferation of MCF-7 cell were detected by MTT test. Results The expression level of hTERT was obviously lower in transfected MCF-7 cell than those in control. Exogenous BRCA1 may inhibit proliferation in MCF-7 cell. Conclusion BRCA1 gene may inhibit expression of hTERT gene and proliferation of MCF-7 cell, which plays important part in the carcinogenesis of breast cancer.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2005年第9期853-855,共3页
Journal of Third Military Medical University
