摘要
目的建立人巨细胞病毒(HumanCytomegalovirus,HCMV)小鼠急性间质性肺炎模型,为HCMV感染性疾病的研究及抗HCMV药物的筛选提供实验基础。方法采用HCMVAD169株感染Balb/c(Baggalbino/c)小鼠,观测小鼠呼吸、体重变化,取外周血检测HCMV特异性抗体,取肺组织进行病毒分离培养、病理学检查及聚合酶链反应(Polymerasechainreaction,PCR)检测。结果HCMV感染组小鼠较对照组呼吸频率增加,体重减轻(P<0.01),实验室检查指标符合间质性肺炎形态学及病毒学改变。结论HCMV可致Balb/c小鼠产生间质性肺炎。
Aim To provide experimental basis to study Human Cytomegalovirus(HCMV)infected disease and filtrate anti-virus drugs, HCMV acute interstitial pneumonia murine model was established. Methods Balb/c mice were infected with HCMV AD_ 169 strain. The rate of breath and weight of mice were observed, specific anti-HCMV antibody in serum was detected by ELISA, and viral isolation, Polymerase chain reaction(PCR) and pathological examination of lung tissue were performed aswell. Results Compared with the control group, the rate of breath of mice in the group infected with HCMV increased, while their weight decreased (P<0.01). The results of lab detection were consistent with morphologic and virological alteration of HCMV interstitial pneumonia. Conclusion HCMV can infect Balb/c mice with interstitial pneumonia.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2005年第5期632-635,共4页
Chinese Pharmacological Bulletin
关键词
巨细胞病毒感染
间质性肺炎
小鼠模型
cytomegalovirus infection
interstitial pneumonia
murine model
