摘要
目的:建立和优化胃癌蛋白质组研究的方法系统,并分析人胃癌细胞SGC7901-asCOX2及SGC7901-pcDNA(用COX-2反义重组质粒及空载体分别转染胃癌细胞系SGC7901得到的两个细胞系)表达的蛋白质组,并建立二者之间的差异表达谱.方法:用固相pH梯度双向凝胶电泳分离SGC7901-asCOX2及SGC7901-pcDNA细胞总蛋白,银染显色,Melanie32-D软件分析两种细胞蛋白质组的表达差异.结果:获得了分辨率和重复性均较好的双向电泳银染图谱.图像分析发现6个蛋白点只在SGC7901-pcDNA细胞中检测到表达,4个点只在SGC7901-asCOX2细胞中检测到表达.与SGC7901-pcDNA细胞相比,在SGC7901-asCOX2细胞中有10个蛋白质点表达下调,6个蛋白点表达上调.结论:人胃癌细胞SGC7901-asCOX2及SGC7901pcDNA表达的蛋白质组有明显差异.
AIM: To establish and optimize the methods for proteome research, and to comparatively analyze the proteomes of human SGC7901-asCOX2 cells and SGC7901-pcDNA cells. METHODS: A series of methods, including immobilized pH gradient two-dimensional polyacrylamide gel electro-phoresis (2DE), silver staining, and Melanie 3 2-D analysis software, were used to analyze the difference of protein expression between SGC7901-asCOX2 cells and SGC7901-pcDNA cells. RESULTS: The 2DE pattern with good resolution and reproducibility was obtained. After silver staining, the 2DE image was analyzed by Melanie 3 2-D software. The results showed that 6 protein spots were exclusively detected in SGC7901 -pcDNA cells, and 4 spots were detected in SGC7901-asCOX2 cells. Compared to SGC7901-pcDNA cells, there were 10 protein spots downregulated in SGC7901-asCOX2, and 6 protein spots upregulated. CONCLUSION: There is a significant difference between proteomes of human gastric SGC7901-asCOX2 cells and SGC7901-pcDNA cells.
出处
《世界华人消化杂志》
CAS
北大核心
2005年第7期840-843,共4页
World Chinese Journal of Digestology
基金
国家自然科学基金资助课题
No.39870320~~
