摘要
目的观察骨髓基质干细胞(B M SC s)在体外能否分化为软骨细胞。方法利用高密度细胞球培养体系在含转化生长因子-β1(TG F-β1)的培养基中培养B M SC s21d,用免疫组化甲苯胺蓝染色方法分析培养的B M SC s球中蛋白多糖(软骨细胞分泌的主要基质成份)的表达、用免疫组化和R T-P C R方法分析Ⅱ型胶原(软骨细胞特异分泌的主要胶原蛋白)的表达来评估B M SC s是否分化为软骨细胞。结果TG F-β1作用的B M SC s表达了Ⅱ型胶原和蛋白多糖。结论B M SC s在体外特定的培养条件下可分化为软骨细胞,从而可能成为临床上治疗创伤或骨关节炎所致的软骨缺损所需的合适的自体来源的种子细胞。
Objective To observe chondrogenesis by bone marrow stromal cells ( BMSCs) in special culture medium containing TGF-β1 in vitro. Methods Chondroge nic differentiation of BMSCs in a high density pellet system was evaluated based on the histological staining of proteoglycan using toluidine blue dye-binding method, immunohistochemical and RT-PCR analysis of the expression of cartilage -specific protein collagen Ⅱ. Results BMSCs cultured in the medium containing TGF-β1 expressed collagen Ⅱand proteoglycan. Conclusion Since TGF-β1 can in duce BMSCs differentiation into chondrocytes in vitro, BMSCs may be suitable aut ogenous cells in treatment of cartilage lesions caused by trauma or osteoarthrit is.
出处
《中华创伤骨科杂志》
CAS
CSCD
2005年第5期442-446,共5页
Chinese Journal of Orthopaedic Trauma
基金
国家重点基础研究发展规划(973)项目(2002CB510100)
国家高技术研究发展计划(863计划)资助项目(2003AA205070)
