摘要
背景:脂代谢紊乱是多种疾病的根源,筛选安全有效的降脂组方具有重要的临床价值。目的:探讨葛根素和精氨酸组合物对大鼠脂代谢的影响。设计:分组对照的观察性实验。单位:解放军总医院动物实验中心和生化科。材料:实验于2004-05/08在解放军总医院动物实验中心和生化科完成。将80只Wister大鼠随机分为高、中、低剂量组和对照组,每组雌、雄各10只。方法:通过自由进食观察不同剂量(0、0.93,1.39和1.85g/kg)的葛根素和精氨酸组合物对大鼠的影响。主要观察指标:①血液学指标。②体质量变化和食物利用率。③主要脏器系数的变化。④组织病理学变化。结果:第2周开始两种性别的动物体质量明显低于对照组。0.93g/kg剂量雌性大鼠和对照组血液中胆固醇分别为(1.63±0.20),(1.29±0.23)mmol/L,雄性大鼠和对照组分别为(1.75±0.13)和(1.31±0.19)mmol/L,雌、雄性大鼠分别与对照组比较差异均有显著性意义(F=6.43,6.58,P<0.05)。3个剂量组脂肪系数与对照组比较则显著降低。结论:该组合物可显著降低实验大鼠的体质量、食物利用率、胆固醇和脏器脂肪比值,具有一定的降脂作用。
BACKGROUND:Disturbance of lipid metabolism is responsible for many kinds of diseases,and to screen safe and effective prescription for lipid reduction has clinical significance. OBJECTIVE:To study the influence of puerarin-arginine composition on lipid metabolism in rats. DESIGN:Comparative observing study based on animal model. SETTING:Experimental animal center and department of biochemistry in a military hospital of Chinese PLA. MATERIALS:This study was carried out at the Experimental Animal Center and Department of Biochemistry of the General Military Hospital of Chinese PLA between May and August 2004.Eighty Wistar rats were randomly divided into high dosage,middle dosage,low dosage group and control group with 10 male and 10 female rats each. METHODS:Rats took food freely,and experimental groups were supplemented with puerarine-arginine composition of different dosage (0,0.93, 1.39 and 1.85 g/kg) for observing the influence on rats. MAIN OUTCOME MEASURES:①blood parameters;②change of body mass and utilization rate of food;③parameters of vital organs; ④histopathological changes. RESULTS:From onset of the second week,body mass of both male and female rats in experimental groups were found obviously lower than those in control group.The levels of blood cholesterol in female rats were(1.63±0.20) and(1.29±0.23) mmol/L in 0.93 g/kg dosage group and control group respectively,and were(1.75±0.13) and(1.31±0.19) mmol/L in male rats,and both have statistical significance(F=6.43,6.58,P< 0.05). Fatty parameters of the three experimental groups were found obviously lower as comparing with those of control group. CONCLUSION:Such composition has been proved to be able to exert function in lipid reduction by decreasing body mass,and improving utilization rate of food and proportion of cholesterol and organic fat.
出处
《中国临床康复》
CSCD
北大核心
2005年第15期220-222,共3页
Chinese Journal of Clinical Rehabilitation
基金
解放军总医院成果转化基金资助(03YC08)~~
